PhD with Dr. James Hall

Plasmid-Host Dynamics in the context of Antibiotic Resistance October 2023 - ongoing

• Studying persistence of antimicrobial resistance as a function of plasmid persistence in the host
• Understanding the interaction dynamics of the plasmid and the host genome

Research Project with Dr. Samay Pande

Research Project on evolution of antibiotic resistance August 2022 - June 2023

• Understanding the influence of multiple selection-constrains on the de-novo evolution of antibiotic resistance
• Studying evolution of antibiotic resistance in prey bacteria in the presence of a predator and evolution of escape strategies by the prey. The model system used was of M xanthus (predator) and E coli, P fluorescence and S enterica (prey).
• Experimental coevolution of M xanthus and three prey bacteria - Microbial culture techniques
• Analysis of predatory efficiency of and competition between clones of M xanthus from a population mixing evolution experiment
• Standardisation of NGS based Frequency Analysis of M xanthus and E coli clones

Masters Thesis with Dr. Frederic Bertels

Studying the dynamics of REPIN movement in bacterial genomes March 2021 - June 2022

• Understanding movement patterns and duplication rate of REPIN Transposable Elements in Pseudomonas genome sequences
• Creating and testing of REPIN Clusterer - a Bioinformatics pipelines in Python Programming Language
• Developed innovative computational method of clustering REPIN Sequences together based on orthology
• Established events of duplication of REPIN Sequences based on the Clusters and calculated their rate of duplication.
• Critical analysis of potential theories on movement of REPIN within host genome
• Automated BLAST searches, isolated genes, compared genes, built, compared and analysed phylogenetic trees, curated computer code and datasets

iGEM 2020 Project

Beta-cells in Gut Producing Insulin (BIG-PIE) February 2020 - October 2020

• A new method of providing insulin to patients with diabetes
• Helped Brainstorm project ideas during the nascent stage
• Helped with building the Project Website
• Contributed to Outreach and Science Communication by conducting seminars for School students
• Gold Medal Winning Project at The Giant Jamboree, iGEM 2020

iGEM 2019 Project

Ecoli Learns Suboptimal Acclimatisation (ELSA) February 2019 - October 2019

• Synthetically modifying Ecoli to grow optimally at 4 degrees Celsius
• Helped manage the Wet Lab experiments by performing Growth Curve experiments to analyse the effect of our Gene of Interest
• Helped the Outreach and Integrated Human Practices team by talking to people in the industry regarding our product.
• Silver Medal Winning Project at The Giant Jamboree, iGEM 2019